Skip to main content
. Author manuscript; available in PMC: 2022 Mar 25.
Published in final edited form as: Dev Cell. 2015 Feb 5;32(5):546–560. doi: 10.1016/j.devcel.2014.12.013

Figure 6. miR-107 is required to control dicer and miR-9 expression across the hindbrain ventricular zone.

Figure 6

(A–B) Expression of dicer and miR-9 in hindbrain cross-sections of MOctrl and MO107a injected embryos at 48 and 60 hpf. Area inside of the gray dashed line indicates the hindbrain area; black stars in (A) show dicer and miR-9 positive areas while black arrowheads show dicer and miR-9 low expression. Black arrowheads in (B) show dicer and miR-9 ectopic expression. Dorsal is up. (C) qRT-PCR of dicer mRNA and the indicated miRNAs in MO107a embryos at 60 hpf. Levels are relative to MOctrl embryos. Error bars represent the ± SD between three independent experiments (n~20 embryos each). *p<0.05. (D) Northern blot analysis of total RNA extracted from 60 hpf embryos injected with MO107a, MOctrl or human DICER mRNA The images are representative of two independent experiments. (E) Schematic representation of the effect of MO107a on dicer (purple) and miR-9 (yellow) pattern expression at 48 and 60 hpf embryos. Scale bars= 50μm.