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Western blot detection of recombinant SARS-CoV-2 spike S1 + S2 subunits bound to S. pneumoniae biofilm (using whole bacterial cell lysate). The blots were individually probed with spike S1 and S2 antibodies. The predicted molecular masses of recombinant spike S1, S2 ECD, and S1 + S2 proteins are 76, 59, and 134 kDa, respectively. Lane 1: Control of S. pneumoniae only; lane 2: S. pneumoniae incubated with S1 + S2 subunits. The results of biological duplicate experiments are shown.
