Figure 6.

IAA-producing strain Azospirillum baldaniorum Sp245 colonizes leaf tissue through stomata. Confocal microscopy showing orthogonal view of (A,B) wild-type and (C) axr1-3 leaf tissue inoculated with Azospirillum baldaniorum Sp245. Note that panels A and B present the z-axis inside the stomata and at the cell surface, respectively, for comparison. The microscopy channels are blue (calcofluor white), green (SYBR Gold DNA), and red (chlorophyll autofluorescence). White arrows indicate bacteria location based on the size of the DNA-stained spots. Bacteria are shown as green spots that are smaller in size to plant nuclei.