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. Author manuscript; available in PMC: 2022 Mar 25.
Published in final edited form as: Methods Mol Biol. 2020;2166:121–144. doi: 10.1007/978-1-0716-0712-1_7

Fig. 3.

Fig. 3

Comparison of MCP-FP variants in mammalian cells. Images are maximum intensity projections from HeLa Flp-in cells transfected with the indicated NLS-MCP-FP variant constructs. Top panels: Images are from cells expressing the indicated NLS-MCP-FP variants without RNA reporter (parental HeLa H9 AAVS1-Tat cell line). Middle panels: Images are from cells expressing the indicated NLS-MCP-FP variant in HeLa H9 AAVS1-Tat cell line expressing the HIV-1 MS2×64 reporter. Bottom panels: Images are smFISH signals obtained with probes against the MS2 tag. Scale bar 5 μm. Contrast adjustment is identical for all images of the top and middle panels