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. Author manuscript; available in PMC: 2022 Mar 25.
Published in final edited form as: Methods Mol Biol. 2020;2166:121–144. doi: 10.1007/978-1-0716-0712-1_7

Fig. 4.

Fig. 4

Schematic representation of pIntro-MS2×64 RNA reporter construct. (a) Generation of the MS2×64 RNA tag. The MS2×32 stem-loop sequence with low-affinity binding to MCP is multimerized to generate repeats of 64 and 128 stem-loops. (b) Schematic of the pIntro-MS2×64 reporter construct. The striped box represents the MS2 repeat; the green spot represents the GFP fused to MCP (blue); the orange oval represents RNAPII with the nascent RNAs. LTR The HIV-1 long terminal repeat; SD1 The major HIV-1 splice donor; SA7 The last splice acceptor; Ψ Packaging sequence; RRE Rev-responsive element