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Branched chain DNA (bDNA) assay for detecting target sequences on dsDNA: (A) Hybridization of two different target probes to dsDNA after melting; (B) Hybridization of the pre-amplifier to the free nucleotides of the two adjacent target probes; (C) Hybridization of identical amplifier molecules to the pre-amplifier; (D) Binding of labelled probes to the amplifier molecules. More information on the graphical elements is given in the box within the figure.
