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CRISPR/dCas9 in genome visualisation. (A) dsDNA detection. dCas9 binds to PAM and sgRNA. dCas9 is fused to a single fluorescent protein or conjugated to multiple fluorescent proteins through Sun-Tag. (B) RNA detection. As in A., but the PAM is extended by an oligonucleotide (PAMmer). More information on the graphical elements is given in the box within the figure.
