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. 2022 Mar 8;14(3):557. doi: 10.3390/v14030557

Figure 5.

Figure 5

Simplified schematic presentation of CRISPR-Sunspot system: (A) Transduction of a cell line with a lentivirus expressing TRE3G-dCas9-24xGCN_V4; (B) The transduced cells are with a second lentivirus expressing scFv-sfGFP lentivirus; (C) Co-transfection of two plasmids of the double-transduced cells; the first coding for two sets of three different sgRNAs each, the second plasmid expresses the PAMmers; (D) Detection and visualisation of the target sequence. sgRNAs and PAMmer bind to the target sequence allowing recruitment of Sun-tagged Cas9. More information on the graphical elements is given in the box within the figure.