Figure 2.

Individualization defects of Z3-5009 mutant visualized by electron microscopy. Cross-sectioned cysts of WT testis show individualized cysts (a); each of the control cysts contains pairs of axonemes and mitochondria derivatives enclosed by individual plasma membrane. Cross-sectioned cysts from Z3-5009 mutant show that individualization does not occur (c); numerous axonemes and mitochondria are enclosed within single membrane and surrounded by a large amount of cytoplasm, and the axonemes are not tightly juxtaposed to the mitochondria. Cross-sectioned cysts of flies that express the Pif1A-RI-GFP transgene and are homozygous for Z3-5009 (P[Pif1A-RI-GFP/CyO3; Z3-5009/Z3-5009]) show that the Z3-5009 phenotype is rescued—after individualization tightly juxtaposed axoneme/mitochondria pairs are surrounded by separate membranes (b). Bar 1 µm.