Figure 4.

Activated caspase activity is normal in the Z3-5009 mutant. Activated caspases (green) are present throughout cysts in the Z3-5009 mutant (b), consistent with Oregon R (a). In control cysts, caspases accumulate in the cystic bulges ((a), white arrowhead) and waste bags ((a), yellow arrowhead). No cystic bulges ((b), white arrowhead) or waste bags ((b), yellow arrowhead) are seen in the Z3-5009 mutant, consistent with the observation that actin cones do not progress away from nuclei. Phalloidin staining, red; DAPI staining, blue. Bar 100 µm.