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Pif1A overview and qPCR. Transcript map of the five predicted Pif1A transcripts (a). Exons of each transcript are shown in red; Pif1A-RI is the transcript used for rescue and targeting in RNAi. The SNP thought to be responsible for the phenotype is 45 bp downstream from the last codon of the Pif1A-RI mRNA transcript (b); it is a C>T transition. Relative Pif1A-RI mRNA expression levels in male testes (c); expression is decreased in Z3-5009 mutant. Error bars represent ± standard error.
