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. 2022 Mar 10;23(6):3011. doi: 10.3390/ijms23063011

Figure 9.

Figure 9

Spermatid individualization in bamGal4>Pif1A-RNAi testis and fertility assays. Actin cones are arrested with nuclear bundles in bamGal4>Pif1A-RNAi testis (a), consistent with the Z3-5009 mutant. Specifically, testes from these flies contained arrested actin cones associated with spermatid nuclei ((a), green arrowhead) or F-actin sleeves ((a), blue arrowhead) and no cystic bulges/waste bags are observed ((a), white arrowhead). Phalloidin staining, red; DAPI staining, blue. A small minority of the testes of this genotype appear roughly WT as explained in the text. Fertility assays (b)—all of the listed Drosophila lines represent males that were crossed with female virgin Oregon R flies; P[Pif1A-GFP], homozygote P[Pif1A-RI-GFP]. Numbers in parentheses refer to sample size. Error bars represent ± standard error. Bar 100 µm.