Figure 2.

Isolation of EPB cells from IJs (A) by Lucskai’s bleach method. Infective dauer juveniles, either from the soil, or insect cadaver, are surface sterilized in HOCL, put in a sterile physiological salt solution in a sterile Petri dish, cut into pieces, and μL volumes from the saline solution are dropped in agar (LBA) media, (C) seeded, and incubated at 25 °C for one day. Fresh colonies are picked and transferred to an indicator (LBTA) plate (B) where the Phase I DPN cells can be unambiguously recognized and cloned. (For details, see Appendix A).