KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| α-Tubulin | Sigma | Cat #T16199 |
| ACSS2 | Cell Signaling Technology | Cat #3658, clone D19C66, lot 2 |
| ACLY | Proteintech | Cat #15421–1-AP, lot 00040639 |
| Histone H3 | Abcam | Cat #ab1791 |
| Histone H4 | Millipore | Cat #05–858 |
| FASN | Cell Signaling Technology | Cat #3189, lot 2 |
| Citrate Synthase | Cell Signaling Technology | Cat #14309, clone D7V8B, lot 1 |
| Lamin A/C | Cell Signaling Technology | Cat #2032 |
| OGDH | Proteintech | Cat #1512–1-AP |
| SUCLA2 | Proteintech | Cat #12627–1-AP |
| SUCLG1 | Proteintech | Cat #14923–1-AP |
| Acetylated lysine (pan Kac) | Cell Signaling Technology | Cat # 9441S, lot 14 |
| Pan anti propionyllysine (pan Kpr) | PTM Biolabs | Cat #PTM-201 |
| Anti-HA magnetic beads | Thermo-Fisher Scientific | Cat #88837 |
| Chemicals | ||
| Acetyl coenzyme A lithium salt | Sigma-Aldrich | Cat #A2181 |
| DL-β-Hydroxybutyryl coenzyme A lithium salt | Sigma-Aldrich | Cat #H0261 |
| Butyryl coenzyme A lithium salt hydrate | Sigma-Aldrich | Cat #B1508 |
| Coenzyme A trilithium salt | Sigma-Aldrich | Cat #C3019 |
| 2-Butenoyl coenzyme A lithium salt | Sigma-Aldrich | Cat #C6146 |
| Glutaryl coenzyme A lithium salt | Sigma-Aldrich | Cat #G9510 |
| DL-3-Hydroxy-3-methylglutaryl coenzyme A sodium salt hydrate | Sigma-Aldrich | Cat #H6132 |
| Isovaleryl coenzyme A lithium salt hydrate | Sigma-Aldrich | Cat #I9381 |
| Malonyl coenzyme A tetralithium salt | Sigma-Aldrich | Cat #63410 |
| n-Propionyl coenzyme A lithium salt | Sigma-Aldrich | Cat #P5397 |
| Succinyl coenzyme A sodium salt | Sigma-Aldrich | Cat #S1129 |
| [13C6,15N2]-Vitamin B5 (calcium pantothenate-13C6,15N2]) | Isosciences | Cat #5065 |
| [15N2,13C5]-Glutamine | Cambridge Isotope Laboratories | Cat #CNLM-1275-H-PK |
| [13C6]-Glucose | Cambridge Isotope Laboratories | Cat #CLM-1396–1 |
| [13C5,15N1]-Valine | Cambridge Isotope Laboratories | Cat #CNLM-422, lot PR-22329 |
| [13C6,15N1]-Leucine | Cambridge Isotope Laboratories | Cat #CNLM-281, lot PR-22831 |
| [13C6]-Isoleucine | Cambridge Isotope Laboratories | Cat #CLM-2248, lot PR-21540 |
| [13C3]-Propionate | Cambridge Isotope Laboratories | Cat #CNLM-1865, lot PR30847/09249SP-1 |
| Propionic anhydride-d10 | Sigma-Aldrich | Cat #615692 |
| Nicotinamide | Sigma-Aldrich | Cat #N0636 |
| Trichostatin A | A.G Scientific | Cat #T-1052 |
| Trichloroacetic acid (TCA) | Sigma-Aldrich | Cat #T6399 |
| Sequencing grade trypsin | Promega | Cat #V5111 |
| Experimental Models: Cell Lines | ||
| Acly−/− mouse embryonic fibroblasts (Clone: PC9) | K.E. Wellen Lab (Zhao et al., 2016) | NA |
| HepG2 | ATCC | Cat. #HB-8065 |
| Murine pancreatic adenocarcinoma (KPC, Clone: 2838c3) | B.Z. Stanger Lab (Li et al., 2018) | NA |
| Hepa1c17 | ATCC | Cat. #CRL-2026 |
| Brown preadipocytes | P. Seale Lab (Harms et al., 2014) | NA |
| Murine liver cancer Aclyf/f (Clone: D42) | K.E. Wellen Lab, this paper | NA |
| Murine liver cancer Acly−/− (Clone: D42C4) | K.E. Wellen Lab, this paper | NA |
| Murine preadipocytes (Clone: 5A) | K.E. Wellen Lab (Fernandez et al., 2019) | NA |
| Phoenix-AMPHO | ATCC | Cat. #CRL-3213 |
| Plasmids | ||
| pMXS-3XHA-EGFP-OMP25 | Addgene (Chen et al., 2016) | Cat. #83356 |
| Software and Algorithms | ||
| Tracefinder v4.1 | Thermo Scientific | |
| FluxFix v1.0 | http://www.flufix.science (Trefely et al., 2016) | NA |
| Graphpad PRISM v8 | GraphPad | |
| EpiProfile 2.0 software | https://github.com/zfyuan/EpiProfile2.0_Family (Yuan et al., 2018) | NA |
| Adobe Illustrator software v23.0.1. | Adobe | NA |