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. 2022 Mar 17;14(3):220. doi: 10.3390/toxins14030220

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Optimization of icELISA experimental conditions and establishment of the icELISA standard curve: (a) selection of different combinations of ZEN mAb and antigen; (b) the effects of methanol contents on the sensitivity of the icELISA (insets indicate the fluctuations of Amax/IC50 as a function of methanol contents); (c) the effects of ionic strengths on the sensitivity of the icELISA (insets indicate the fluctuations of Amax/IC50 as a function of ionic strengths); (d) the effects of pH values on the sensitivity of the icELISA (insets indicate the fluctuations of Amax/IC50 as a function of pH values.