Table 1.
The sensitivity (IC50 values) and specificity (CR values) of four mAbs against ZEN.
| Compound | 2B6 | 4D9 | 1A10 | 4G8 | ||||
|---|---|---|---|---|---|---|---|---|
| IC50 (μg/L) a,b | CR (%) c | IC50 (μg/L) a,b | CR (%) c | IC50 (μg/L) a,b | CR (%) c | IC50 (μg/L) a,b | CR (%) c | |
| ZEN | 10.38 | 100 | 17.23 | 100 | 19.87 | 100 | 27.05 | 100 |
| α-ZAL | 682.89 | 1.52 | 1057.06 | 1.63 | 296.13 | 6.71 | 379.92 | 7.12 |
| β-ZAL | 810.94 | 1.28 | 1276.30 | 1.35 | 299.25 | 6.64 | 390.33 | 6.93 |
| α-ZOL | 393.18 | 2.64 | 602.45 | 2.86 | 176.62 | 11.25 | 231.99 | 11.66 |
| β-ZOL | 567.21 | 1.83 | 857.21 | 2.01 | 243.51 | 8.16 | 317.49 | 8.52 |
| ZON | 243.09 | 4.27 | 353.07 | 4.88 | 103.12 | 19.12 | 132.86 | 20.36 |
| Aflatoxin B1 | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 |
| Deoxynivalenol | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 |
| T-2 toxin | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 |
| Ochratoxin | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 | >10,000 | <1 |
Note. a The data were calculated from triplicate assays. The average coefficient of variation (CV) was below 10%. b The compound standard solution was prepared in 70% methanol-PBS (phosphate buffer saline, 0.01 M, pH 7.4) (7:3, v/v). c The data were calculated using the CR values of the mAbs against ZEN as 100%.