Table 3.
Accuracy and precision measurements of the icELISA for ZEN.
| Samples | ZEN Spiked (μg/L) |
Inner Batch | Among Batches | ||||
|---|---|---|---|---|---|---|---|
| Measured Value (μg/L) a |
Recovery (%) b |
CV (%) a,c |
Measured Value (μg/L) a |
Recovery (%) b |
CV (%) a,c |
||
| Maize meal d | 10 | 10.34 ± 1.13 | 103.4 | 13.7 | 10.21 ± 0.98 | 102.1 | 13.5 |
| 20 | 19.34 ± 1.56 | 96.7 | 12.5 | 19.48 ± 1.36 | 97.4 | 12.4 | |
| 40 | 37.44 ± 2.21 | 93.5 | 11.3 | 37.68 ± 1.86 | 94.2 | 12.4 | |
| 80 | 72.16 ± 2.87 | 90.2 | 13.6 | 72.41 ± 2.11 | 90.5 | 11.8 | |
| Wheat meal e | 10 | 9.77 ± 1.42 | 97.7 | 12.9 | 9.81 ± 0.86 | 98.1 | 13.5 |
| 20 | 18.82 ± 173 | 94.1 | 13.4 | 18.92 ± 1.28 | 94.6 | 13.3 | |
| 40 | 36.91 ± 2.43 | 92.3 | 12.6 | 37.24 ± 1.83 | 93.1 | 12.6 | |
| 80 | 71.84 ± 3.04 | 89.8 | 11.3 | 72.72 ± 2.41 | 90.9 | 11.7 | |
| Pig feed f | 10 | 10.67 ± 1.41 | 106.7 | 14.2 | 10.47 ± 1.22 | 104.7 | 14.6 |
| 20 | 20.46 ± 1.87 | 102.3 | 13.8 | 19.74 ± 1.41 | 98.7 | 13.2 | |
| 40 | 38.64 ± 2.61 | 96.6 | 12.4 | 36.72 ± 1.74 | 91.8 | 12.7 | |
| 80 | 73.84 ± 3.56 | 92.3 | 11.5 | 71.44 ± 2.46 | 89.3 | 13.8 | |
Note. a Measured values are presented as mean of six replicates. b recovery (%) = (concentration measured/concentration spiked) × 100. c CV indicates the coefficient of variation. d Peeled powder of common yellow maize. e Bran-removed powder of common red wheat. f Complete formula feed of young pigs.