Table 1.
PCR primers used for the detection of virulence genes.
| Gene | Virulence Factor | Primer Name | Oligonucleotide Sequence (5′ to 3′) | Product Size (bp) |
|---|---|---|---|---|
| asa1 | aggregation substance | ASAfw ASArw |
GCACGCTATTACGAACTATGA TAAGAAAGAACATCACCACGA |
375 |
| cylA | cytolysin | CYTfw CYTrw |
TATGACAATGCTTTTTGGGAT AGATGCACCCGAAATAATATA |
213 |
| gelE | gelatinase | GELfw GELrw |
ATAGACAATGCTTTTTGGGAT AGATGCACCCGAAATAATATA |
213 |
| hyl | hyaluronidase | HYLfw HYLrw |
ACAGAAGAGCTGCAGGAAATG GACTGACGTCCAAGTTTCCAA |
276 |
| esp | surface protein | SPfw SPrw |
AGATTTCATCTTTGATTCTTGG AATTGATTCTTTAGCATCTGG |
688 |