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. 2022 Mar 21;23(6):3380. doi: 10.3390/ijms23063380

Figure 6.

Figure 6

Responses of the mpl1, lip1, and mpl1lip1 plants to freezing and heat stresses. (A) Electrolyte leakage indictive of damage of biological membranes caused by freezing temperatures. NC, non-cold acclimated; AC, cold acclimated. (B) Hypocotyl elongation of mpl1, lip1, and mpl1lip1 in response to heat stress. Seeds were sown in two rows on vertical MS medium plates and incubated at 22 °C for 36 h in darkness to ensure uniform seed germination and initial hypocotyl elongation. There were 14–20 seeds for each genotype per MS medium plate. The germinated seedlings were treated at 45 °C for 0 or 1 h and allowed to grow in darkness in a growth room at 22 °C for an additional 3 days. Data are means ± sd (n = 18 in (A) (n = number of detached leaves), 4 (Control), 9 (Heat) in (B) (n = number of MS medium plates)). One-way ANOVA (Tukey test) was performed, and significant difference was indicated by different lowercase letters (p < 0.05).