Figure 2.

Exosomes extracted from plasma of all healthy subjects (20) and NAFLD-affected patients (95) with moderate (38) or severe (57) hepatic steatosis at recruitment time (T0) and after treatment (T2): (A) representative Western Blotting of FZD7, ALIX, and HSP-70 (molecular mass markers are indicated on the right); (B) semi-quantitative estimation, by Western blotting analysis and densitometry of protein bands, of relative FZD7 expression level; (C) quantitative analysis of FZD7 by ELISA assay. For semiquantitative analysis, the same total protein content (20 µg) was loaded and FZD7 bands were evaluated upon normalization with the corresponding housekeeping ALIX protein band for each sample. * p < 0.05 and ** p < 0.001 versus control.