Figure 3.

Effect of HRW and EO extracts from E. globulus leaves on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The cells were treated with 0.32 mg/mL EO or 12.5 μg/mL HRW in the presence or absence of 1 μg/mL LPS, for 24 h. The cells treated with the medium alone were used as the CTRL. The NO production was determined in the cell culture supernatants using the Griess reagent. The results were expressed as the percentage (%) of nitrite production relative to the LPS; they represent the mean ± SEM of at least three independent experiments performed in triplicate. The statistical analysis was performed by one-way ANOVA followed by Dunnett’s and Sidak’s multiple comparison tests. ** p < 0.01 and **** p < 0.0001: significantly different compared to the CTRL. # p < 0.05 and ## p < 0.01: significantly different compared to the LPS.