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. 2022 Mar 3;14(3):561. doi: 10.3390/pharmaceutics14030561

Figure 8.

Figure 8

Effect of HRW and EO extracts from E. globulus leaves on MMP-1, MMP-9 and MPP-13 activity. In a cell-free system, MMP-1, MMP-9 or MMP-13 enzymes were pre-incubated with EO (0.16 mg/mL), HRW (0.8 μg/mL) or ethylenediamine tetraacetic acid (EDTA) (0.14 mM) for 30 min at 37 °C, followed by the addition of MMP fluorogenic substrate. The enzymatic activity results were expressed as the percentage (%) of enzyme inhibition relative to the activity determined in the CTRL; the results represent the mean ± SEM of at least three independent experiments. The statistical analysis was performed by one-way ANOVA followed by Dunnett’s multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001: significantly different compared to the CTRL.