Table 2.
Chemical characterization of the hydrodistillation residual water extract (HRW) from E. globulus leaves by high-performance liquid chromatography with a photodiode array detector coupled to mass spectrometry with electrospray ionization (HPLC-PDA-ESI/MSn).
| Peak * | Rt (min) | λmax (nm) |
HPLC-ESI-MSn [m/z (Relative Abundance, %)] |
Attempt to Identify | ||
|---|---|---|---|---|---|---|
| Precursor Ion [M-H] |
MS2 | MS3 | ||||
| 1 | 3.24 | 234sh, 238, 246, 257 | 633(100) | 301(100), 275 (16), 249 (15) | 284 (34), 257 (80), 229 (100), 201 (15), 185 (55), 173 (13) | HHDP galloyllglucose |
| 2 | 4.38 | 234sh, 238, 246, 270 | 169(100) | 125(100) | 125 (46), 107 (13), 97(100), 81 (96), 79 (28), 69 (12) | Gallic acid |
| 3 | 5.63 | 231sh, 234sh, 238, 246, 277 | 633(100) | 301(100) | 284 (61), 257(100), 229 (63), 213 (11), 201 (31), 185 (70) | HHDP galloyllglucose |
| 4 | 12.39 | 231sh, 238, 246, 265 | 783(100) | 935 (24), 765(100), 613 (12) | 721 (12), 613 (90), 597(100), 533 (13), 443 (11), 427 (29), 401 (20), 399 (17), 325 (19), 301 (58), 275 (63), 273(15), 231 (15), 229 (12) | bis-HHDP-glucose |
| 5 | 17.14 | 231sh, 238, 246sh, 256, 289sh | 353(100) | 233(100) | 205(100) | 3-Caffeoyl-quinic acid |
| 6 | 18.36 | 229sh, 238, 246sh, 252, 288sh, 299, 325 | 431(100) | 385(100), 223 (16) | 295 (11), 223(100), 205 (57), 161 (48), 153 (57), 151 (12) | Sinapoyl-hexoside |
| 7 | 18.93 | - | 191(100) | 173 (90), 127(100), 85(65) | 109(100), 85 (55) | Quinic acid |
| 8 | 18.93 | 231sh, 238, 246sh, 252, 292sh, 299sh, 325 | 353(100) | 191(100) | 127(100), 172 (60), 85(55) | 5-Caffeoyl-quinic acid |
| 9 | 20.03 | 232sh, 238, 246, 259, 330sh | 461(100) | 415(100) | 269(100), 247 (14), 161 (33) | Not identified |
| 10 | 21.13 | 234sh, 238, 246, 259, 368sh | 1085(100) | 783 (12), 765(100) | 613 (58), 597 (61), 595 (14), 427 (21), 399 (27), 383 (19), 301(100), 275 (51), 273 (14), 259 (10), 231 (14), 229 (21) | Cornusiin B or eucalbanin A |
| 11 | 21.52 | 232sh, 238, 246, 259, 371sh | 1085(100) | 1069 (25), 765(100), 755 (30), 451 (12) | - | Cornusiin B or eucalbanin A |
| 12 | 22.10 | 226sh, 234sh, 238, 246, 258sh, 265, 366sh | 451(100) | 313 (40), 271 (84), 211(100), 169 (92), 151 (13) | 168(100), 124 (12) | Galloyl-glucose ester |
| 13 | 22.68 | 233sh, 238, 246, 261, 299sh, 358sh | 1253(100) | 1074 (47), 971(100), 781 (39), 640 (79) | - | Punicalin derivative |
| 14 | 25.25 | 233sh, 238, 246, 257, 269sh, 309sh, 353sh, 362, 367sh | 565(100) | 550 (78), 549 (12), 519 (17), 419(100), 405 (60). 401 (44), 386 (28), 373 (21), 355 (11), 233 (18), 202 (21), 187 (19) | 404(100), 373 (25) | Not identified |
| 15 | 26.52 | 231sh, 234sh, 238, 246, 262, 292sh, 352sh | 275(100) | 257(100), 247 (13), 231 (14), 229 (31), 203 (21) | - | Not identified |
| 16 | 34.34 | 226sh, 234sh, 238, 243sh, 246sh, 258sh, 268, 356sh | 497(100) | 331(100), 169 (79) | 169(100), 125 (22) | Eucaglobulin |
| 17 | 35.48 | 231sh, 234sh, 238, 246sh, 255, 265sh, 346sh, 364, 380sh | 477(100) | 315(100), 300 (20) | 300(100) | Methylellagic acid hexose |
| 18 | 38.16 | 232sh, 234sh, 238, 246, 258, 265sh, 354, 381sh | 477(100) | 301(100) | 273 (15), 257 (12), 179(100), 151 (85) | Quercetin-O-glucuronide |
| 19 | 39.20 | 227sh, 238, 239sh, 242sh, 244sh, 246sh, 260, 266sh, 295sh, 354, 381sh | 609(100) | 301(100), 271 (35), 255 (12) | 273 (12), 239 (13), 179(100), 151 (79) | Quercetin 3-O-rutinoside |
| 20 | 43.08 | 234sh, 238, 246sh, 255, 267sh, 306sh, 351sh, 367, 382sh | 301(100) | 284 (55), 257 (84), 245 (13), 229(100), 201 (22), 185 (78) | 212 (13), 201 (69), 185(100), 173 (34), 157 (34), 145 (34) | Ellagic acid |
| 21 | 45.25 | 229sh, 232sh, 238, 241sh, 246sh, 257, 267sh, 301sh, 351, 381sh | 447(100) | 301(100) | 273 (19), 179(100), 151 (81) | Quercetin3-O-rhamnoside |
| 22 | 46.94 | 231sh, 234sh, 238, 242sh, 246sh, 257sh, 261, 346 267sh, 293sh, 357sh, 380sh, 412sh, 420sh, 446sh, 466sh, 486 | 461(100) | 285(100), 173 (14) | - | Luteolin 7-O-glucuronide |
* The number of the peaks in this table corresponds to the peaks indicated in Figure 1. MS2 = 1st generation product ion spectra; MS3 = 2nd generation product ion spectra.