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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Cytotherapy. 2021 Dec 23;24(3):282–290. doi: 10.1016/j.jcyt.2021.10.007

Figure 2. Combinatorial Targeting with CLL-1 CAR and CD123 CARs in vitro.

Figure 2.

2A. Schematic figures of CLL-1 CAR, CD123 CAR.CD28z constructs. 2B. CAR expression in single and co-transduced T cells shown on day 3 after transduction. 2C. T cell expansion of NTR, CLL-1 CAR, CD123 CAR. CD28z, single and doubly (combinatorial) transduced cells in culture. 2D. The residual counts of tumor cells in THP-1 (left), HL-60 (middle), MOLM13 (middle), and KGA1 (right) after co-culture at 72 hours E:T (1:4). (NTR: Non-Transduced). 2E. pCD3z expression in single vs combinatorial targeting when exposed to KG1a. 2F. Total CD3+ T cell counts after 72 hrs co-culture with KG1a at E:T =1:4 (P>0.05). Residual CAR T cell counts with CLL-1 CAR +/− CD123 CAR.CD28 (NTR:Non Transduced).