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Increasing the level of one-carbon metabolites in the ECM-detached condition. (a) Overall experimental design. (b) The one-carbon metabolites detected in the attached (AT) and 6 days (6D) ECM-detached HeLa cell line. (c) The one-carbon metabolites detected in the attached (AT) and 6 days (6D) ECM-detached MCF-7 cell line. The data are presented as mean ± SEM; ns, not significant; * p < 0.05, *** p < 0.001 and **** p < 0.0001.
