Figure 5.

Anti-proliferative effect of G3^gh2B5M, G3^2B12gh5M, G3^gh4B5V, G3^2B12gh5V, αM, and V on BJ, U-118 MG, and SCC-15 cells after 72 h of incubation, determined with Hoechst 33,342 staining. Medians of the fluorescence signal (expressed as percent of the non-treated control), indicating the cell number in a well, are plotted. The whiskers indicate lower (25%) and upper (75%) quartile ranges. * p ≤ 0.05; Kruskal-Wallis test (against non-treated control), ↕ p ≤ 0.05; Mann-Whitney U test (ester against amide-attached drug-conjugate). ^1,2 Data for G3^2B12gh5M conjugate and αM were reproduced from [2,29], respectively.