Table 10.

Effect of L-Threonine and Mycofix Plus (MP) on serum antioxidant capacity of broilers exposed to Aflatoxin B₁, Cobb 500.

				SOD 1	GPX 2	CAT 3
Treatments	L-Threonine, % of Requirements	MP, g/kg	Aflatoxin B1, 500 µg/kg	u/mL	u/mL	u/mL
T1	100	0	−	14.89	564.29	7.91
T2	100	0	+	14.37	518.98	6.01
T3	100	1	−	13.16	648.57	5.68
T4	100	1	+	14.23	651.32	6.87
T5	125	0	−	16.05	649.48	6.21
T6	125	0	+	13.71	686.27	5.62
T7	125	1	−	15.37	735.98	7.80
T8	125	1	+	14.43	728.32	7.44
Pooled SEM				1.25	69.28	1.37
Main Effects			Levels	Means 4
L-Threonine			100	14.16	595.79 b	6.62
			125	14.89	700.01 a	6.77
Mycofix Plus			0	14.75	604.75	6.44
			1	14.30	691.05	6.95
Aflatoxin B1			+	14.87	649.58	6.90
			−	14.18	646.22	6.48
Main Effects and Interaction Effects				p-values
L-Threonine				NS	*	NS
Mycofix Plus				NS	NS	NS
Aflatoxin B1				NS	NS	NS
L-Threonine × Mycofix Plus				NS	NS	NS
L-Threonine × Aflatoxin B1				NS	NS	NS
Mycofix Plus × Aflatoxin B1				NS	NS	NS
L-Threonine × Mycofix Plus × Aflatoxin B1				NS	NS	NS

^a,b Means within a column with differing superscripts are significantly different at * p < 0.05. NS, p ≥ 0.05. ¹ Superoxide dismutase. ² Glutathione peroxidase. ³ Catalase. ⁴ Means represent 32 pens of chickens with 10 birds per pen (n = 32/group).