Table 2.
The non-synonymous amino acid mutations harbored by the Italian genomes (n = 111) investigated here and non-synonymous amino acid mutations harbored by the 58 Italian genomes collected from the Istituto Superiore di Sanità and from the Italian Scientific Department of the Army Medical Center. The amino acids that resulted converted to a stop codon (*) are also reported.
| Mutation | n/Total | Percentage | n/58 (ISS and Army Medical Center) | Percentage | Target | Gene |
|---|---|---|---|---|---|---|
| P323L | 103/111 | 92.80% | 56/58 | 96.50% | nsp12-RNA-dependent RNA polymerase | ORF1ab |
| D614G | 103/111 | 92.80% | 56/58 | 96.50% | spike | S |
| R203K | 41/111 | 37% | 23/58 | 39.70% | nucleocapsid | N |
| G204R | 41/111 | 37% | 23/58 | 39.70% | nucleocapsid | N |
| D3G | 13/111 | 11.70% | 9/58 | 15.50% | membrane | M |
| L37F | 9/111 | 8.10% | 4/58 | 6.90% | nsp6 | ORF1ab |
| G251V | 8/111 | 7.20% | 2/58 | 3.44% | ORF3a | ORF3a |
| V246I | 4/111 | 3.60% | 3/58 | 5.20% | nucleocapsid | N |
| T175M | 3/111 | 2.70% | 2/58 | 3.44% | membrane | M |
| L5F | 3/111 | 2.70% | 2/58 | 3.44% | spike | S |
| A302V | 2/111 | 1.80% | 2/58 | 3.44% | nsp2 | ORF1ab |
| D218E | 2/111 | 1.80% | 2/58 | 3.44% | nsp3 | ORF1ab |
| S74A | 2/111 | 1.80% | 1/58 | 1.72% | nsp13/helicase | ORF1ab |
| A81V | 2/111 | 1.80% | 2/58 | 3.44% | nsp 15/endoRNAse | ORF1ab |
| Q218R | 2/111 | 1.80% | 2/58 | 3.44% | nsp16/2’-O-ribose methyltransferase | ORF1ab |
| L41F | 2/111 | 1.80% | 2/58 | 3.44% | ORF3a | ORF3a |
| H182R | 2/111 | 1.80% | 2/58 | 3.44% | ORF3a | ORF3a |
| Q12 * | 2/111 | 1.80% | 2/58 | 3.44% | ORF10 | ORF10 |
| P191S | 1/111 | 0.90% | 1/58 | 1.72% | nsp2 | ORF1ab |
| T237I | 1/111 | 0.90% | 1/58 | 1.72% | nsp3 | ORF1ab |
| A496V | 1/111 | 0.90% | 1/58 | 1.72% | nsp3 | ORF1ab |
| A534V | 1/111 | 0.90% | 1/58 | 1.72% | nsp3 | ORF1ab |
| P1159S | 1/111 | 0.90% | / | / | nsp3 | ORF1ab |
| S1285F | 1/111 | 0.90% | / | / | nsp3 | ORF1ab |
| I1413L | 1/111 | 0.90% | / | / | nsp3 | ORF1ab |
| M1547I | 1/111 | 0.90% | / | / | nsp3 | ORF1ab |
| N1785D | 1/111 | 0.90% | 1/58 | 1.72% | nsp3 | ORF1ab |
| A1872V | 1/111 | 0.90% | / | / | nsp3 | ORF1ab |
| V357I | 1/111 | 0.90% | 1/58 | 1.72% | nsp4 | ORF1ab |
| A457V | 1/111 | 0.90% | 1/58 | 1.72% | nsp4 | ORF1ab |
| K285N | 1/111 | 0.90% | / | / | nsp6 | ORF1ab |
| A287S | 1/111 | 0.90% | / | / | nsp6 | ORF1ab |
| S10L | 1/111 | 0.90% | / | / | nsp7 | ORF1ab |
| F49I | 1/111 | 0.90% | / | / | nsp7 | ORF1ab |
| Y149 * | 1/111 | 0.90% | / | / | nsp8 | ORF1ab |
| W154R | 1/111 | 0.90% | / | / | nsp8 | ORF1ab |
| P80S | 1/111 | 0.90% | 1/58 | 1.72% | nsp9 | ORF1ab |
| R285H | 1/111 | 0.90% | 1/58 | 1.72% | nsp12-RNA-dependent RNA polymerase | ORF1ab |
| S363R | 1/111 | 0.90% | 1/58 | 1.72% | nsp12-RNA-dependent RNA polymerase | ORF1ab |
| D893Y | 1/111 | 0.90% | / | / | nsp12-RNA-dependent RNA polymerase | ORF1ab |
| P529L | 1/111 | 0.90% | 1/58 | 1.72% | nsp13/helicase | ORF1ab |
| T31I | 1/111 | 0.90% | 1/58 | 1.72% | nsp14/3’-to-5’ exonuclease | ORF1ab |
| V287F | 1/111 | 0.90% | 1/58 | 1.72% | nsp14/3’-to-5’ exonuclease | ORF1ab |
| P23S | 1/111 | 0.90% | / | / | nsp 15/endoRNAse | ORF1ab |
| S154F | 1/111 | 0.90% | / | / | nsp 15/endoRNAse | ORF1ab |
| K160R | 1/111 | 0.90% | 1/58 | 1.72% | nsp16/2’-O-ribose methyltransferase | ORF1ab |
| A222S | 1/111 | 0.90% | 1/58 | 1.72% | spike | S |
| P681S | 1/111 | 0.90% | 1/58 | 1.72% | spike | S |
| D839Y | 1/111 | 0.90% | / | / | spike | S |
| M1T | 1/111 | 0.90% | 1/58 | 1.72% | ORF3a | ORF3a |
| D2Y | 1/111 | 0.90% | 1/58 | 1.72% | ORF3a | ORF3a |
| Q57H | 1/111 | 0.90% | 1/58 | 1.72% | ORF3a | ORF3a |
| A110S | 1/111 | 0.90% | / | / | ORF3a | ORF3a |
| G6V | 1/111 | 0.90% | 1/58 | 1.72% | membrane | M |
| W29R | 1/111 | 0.90% | 1/58 | 1.72% | ORF7b | ORF7b |
| D22Y | 1/111 | 0.90% | 1/58 | 1.72% | nucleocapsid | N |
| T24N | 1/111 | 0.90% | 1/58 | 1.72% | nucleocapsid | N |
| D103Y | 1/111 | 0.90% | / | / | nucleocapsid | N |
| R185H | 1/111 | 0.90% | 1/58 | 1.72% | nucleocapsid | N |
| L331F | 1/111 | 0.90% | 1/58 | 1.72% | nucleocapsid | N |
| A381V | 1/111 | 0.90% | 1/58 | 1.72% | nucleocapsid | N |
| G50N | 41/111 | 37% | 23/58 | 39.7% | ORF14 (uncharacterized protein) | ORF14 |
| G50E | 1/111 | 0.90% | 1/58 | 1.72% | ORF14 (uncharacterized protein) | ORF14 |
| V32I | 1/111 | 0.90% | 1/58 | 1.72% | ORF14 (uncharacterized protein) | ORF14 |
| R32L | 1/111 | 0.90% | 1/58 | 1.72% | ORF9b | ORF9b |
| L21M | 1/111 | 0.90% | 1/58 | 1.72% | ORF9b | ORF9b |
| Q18H | 1/111 | 0.90% | 1/58 | 1.72% | ORF9b | ORF9b |
| G71S | 1/111 | 0.90% | 1/58 | 1.72% | nsp5 | ORF1ab |