Figure 5.

Quercetin and quercitrin inhibit cell migration through the JNK signaling pathway. (A) RKO cells were treated with fraction 4, quercetin, or quercitrin for 24 h. Total cell lysates were prepared and the levels of p38, p-p38, JNK, p-JNK, ERK, and p-ERK were detected by Western blot analysis. (B) The cells were pretreated with SP600125, SB202190, or U0126 for 2 h and then treated with fraction 4, quercetin, or quercitrin. After 72 h, wound healing was quantified by measuring the mean line of the wound edges. All experiments were performed at least three times. The data are presented as the means ± SD. (C) The cells were pretreated with SP600125 for 2 h and then with fraction 4, quercetin, or quercitrin for an additional 24 h. Total cell extracts were prepared and subjected to Western blot analysis. * p < 0.01.