FIG. 1.

Identification of Cp^r VGS isolates by the PCR method described by Garnier et al. (12). The DNAs of S. oralis ATCC 10557, S. oralis NCTC 11427, S. mitis NCTC 12261, and clinical isolates (S. mitis V1, V2, V3, and V10) were amplified with the oligonucleotide pairs indicated. O, M, and S, the S. oralis-, S. mitis-, and S. sanguis-specific pairs, respectively; Mw, bacteriophage λ DNA digested with EcoRI and HindIII. PCR products were resolved by electrophoresis on a 1.5% agarose-Tris-acetate-EDTA gel that was stained with a 0.5-μg/ml ethidium bromide solution. The sizes (in base pairs) of the PCR products expected for the different species are indicated on the left.