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. 2022 Mar 23;17(1):248–260. doi: 10.1515/biol-2022-0029

Figure 4.

Figure 4

circ-CFH/miR-377-3p axis regulated proliferation, apoptosis, migration, invasion, and glycolysis of hepatocellular carcinoma cells. (a) The interference efficiency of anti-miR-377-3p was checked by the RT-qPCR assay. (b–i) Huh7 and SNU387 cells were divided into five groups: control, si-NC, si-circ-CFH, si-circ-CFH + anti-miR-NC, and si-circ-CFH + anti-miR-377-3p. (b) MTT assay was performed for examining the cell viability of Huh7 and SNU387 cells. (c) The apoptosis rate was shown by Annexin V-FITC/PI staining in Huh7 and SNU387 cells. (d and e) Transwell assay was performed in transfected Huh7 and SNU387 cells. (f–h) The glucose consumption, lactate, and ATP level of transfected Huh7 and SNU387 cells are shown. (i) Western blot analysis was used to show the expression level of GLUT1 in Huh7 and SNU387 cells. **P < 0.01, ***P < 0.001, ****P < 0.0001.