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. 2022 Mar 23;17(1):248–260. doi: 10.1515/biol-2022-0029

Figure 6.

Figure 6

miR-377-3p regulated proliferation, apoptosis, migration, invasion, and glycolysis of hepatocellular carcinoma cells by affecting RNF38. (a and b) The overexpression efficiency of the RNF38 vector was checked by RT-qPCR and western blot analyses in Huh7 and SNU387 cells. (c–j) Huh7 and SNU387 cells were divided into five groups: control, miR-NC, miR-377-3p, miR-377-3p + pcDNA, and miR-377-3p + RNF38. (c) The cell viability of Huh7 and SNU387 cells was determined by the MTT assay. (d) Annexin V-FITC/PI staining was performed to assess the apoptosis of Huh7 and SNU387 cells. (e–f) Transwell assay was used to measure migration and invasion of Huh7 and SNU387 cells. (g–i) The glucose consumption, lactate, and ATP level are shown in Huh7 and SNU387 cells. (j) The expression level of GLUT1 was quantified by western blot analysis in Huh7 and SNU387 cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.