Figure 1.
The molecular diagnostic techniques of schistosomiasis japonica and the year of first report in China. 1 nPCR is characterized by two pairs of primers, inner primer and outer primer; 2 qPCR can quantitatively and qualitatively analyze the initial template of samples by detecting the fluorescence signal corresponding to each cyclic amplification product in real time; 3 The combination of RPA and lateral flow dipstick (LFD) for visual detection. Generally, visualization of control line and the test line is positive, and only the control line is negative; 4 cPCR determined positive or negative results by the size of the gel electrophoresis band; 5 The combination of LAMP reaction with chemical dyes for visual detection. + positive reaction, − negative reaction; 6 Dilute sample or samples DNA to the single molecule level and collect the fluorescence signal of a single reaction unit to achieve the absolute quantitative detection. Black spots: positive reaction units, blank spots: negative reaction units.