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. 2022 Feb 24;11(3):287. doi: 10.3390/pathogens11030287

Table 2.

The target genes and application performance of qPCR assay for schistosomiasis japonica.

Target Sequence No. of GenBank Accession Fragment Size (bp) Detection Limit Sensitivity (%) Specificity (%) Prevalence 1 Specimen Year of Publication
1 NADH I N/D 2 82 1 EPG N/A 3 N/A N/A Human feces 2006 [84]
2 NADH I N/D 75 1 EPG N/A N/A 5.3% Human feces 2009 [85]
3 NADH I N/D 82 5 EPG 100/96.83 100 11.06% of human; 24.73% of bovines Buffalo and human feces 2018 [86]
4 NADH I N/D 82 1 EPG 100 100 51.5% Buffalo feces 2010 [87]
5 NADH I N/D 82 one egg; 14 pg 95.25/94.0 100 95.45% buffalo and Human feces;
gDNA		 2012 [88]
6 NADH I N/D 82 N/A N/A N/A 87.50% Bovines feces 2015 [89]
7 NADH I N/D 82 N/A N/A N/A 90.2% Human feces 2015 [90,91]
8 NADH I N/D 82 N/A N/A N/A N/A Serum, urine and fecal of pig model 2008 [92]
9 NADH I N/D 82 5 EPG N/A N/A N/A Buffalo feces 2009 [93]
10 NADH I N/D 82 N/A N/A N/A 9.21% of rodents; 18.37% of dogs; 6.9% of goats Rodents, dogs and goats feces 2017 [94]
11 NADH I AF215860 75 N/A N/A N/A N/A Organs 2018 [95]
12 COX I N/D 119 N/A N/A N/A N/A Water samples 2019 [96]
13 18S rRNA AYl57226 81 6.15 pg N/A N/A 48.0% gDNA 2008 [97]
14 18S rRNA AY157226 81 10 fg N/A N/A N/A gDNA 2011 [98]
15 18S rRNA AY157226.1 N/D 20 fg N/A N/A N/A gDNA 2011 [99]
16 18S rRNA FJ176682 156 4.3 × 102 copies plasmid; 0.4 fg gDNA; 10 EPG; one cercaria in 10 pooled snails; N/A N/A N/A Plasmids; gDNA; mice feces; snail 2013 [100]
17 18S rRNA AY157226 280 10 fg N/A N/A N/A gDNA 2015 [101]
18 ITS 2 U22167 192 1.42 × 102 copies plasmid; 10 pg gDNA; 10 EPG; 100 100 N/A Snail and mice feces 2011 [102]
19 SjR2 AF412221.1 N/D 2 pg N/A N/A N/A gDNA 2011 [99]
20 SjR2 AF412221 N/D 44.7 copies plasmid N/A N/A N/A Plasmids and sera of rabbit 2014 [103]
21 SjR2 N/D N/D N/A N/A N/A N/A Water samples 2021 [104]
22 SjCHGCS20 FN356222.1 N/D N/D 98.74 100 8.33 Plasma of goat 2020 [22]
23 SjCHGC08270 AY812553 85 half of one cercaria 93.75 N/D N/A N/A 2008 [20]
24 SjCHGC08270 AY812553 85 one cercaria N/A N/A 6.48 Water samples 2011 [105]
25 Sjrh1.0 U92488.1 N/D 2 fg N/A N/A N/A gDNA and sera of mice 2011 [99]
26 Sjrh1.0 U92488.1 N/D 2 fg N/A N/A N/A gDNA and water samples 2011 [106]

1 Prevalence: no. positive/no. examined; 2 N/D: Non-disclosed; 3 N/A: Non-applicable.