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. 2022 Mar 11;14(3):575. doi: 10.3390/v14030575

Figure 2.

Figure 2

The I226R protein reduces the expression of IFNs induced by SeV and poly(I:C): (A–C) 293T cells were transfected with 3 μg I226R-Flag plasmid or EV for 24 h; then, they were infected with SeV (A,B), or were transfected with 1 μg/mL poly(I:C) (C) for 12 h. RT-qPCR and ELISA were performed to examine the expression of IFN-β mRNA and protein levels, respectively. (D,E) 293T cells were transfected with 3 μg I226R-Flag plasmid or EV for 24 h; then, they were infected with SeV for 12 h. Expression levels of IL28 (D) and IL29 (E) were measured by RT-qPCR analysis. (F) 293T cells were transfected with 3 μg EV, NS1(H7N9), or I226R-Flag plasmid for 24 h; then, they were infected with PR8 ∆NS1 (MOI = 1) for 12 h. The supernatant of virus-infected cells was used for plaque assay. All the results are expressed as the means ± standard deviation from three independent experiments. Statistical analysis was performed using the Student’s t-test. ** p < 0.01.