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. 2022 Mar 11;14(3):575. doi: 10.3390/v14030575

Figure 3.

Figure 3

I226R-overexpressing cells have impaired expression of several critical ISGs. (A–E) 293T cells were transfected with 3 μg I226R-Flag plasmid or EV for 24 h; then, they were infected with SeV for 12 h. RT-PCR (A) and RT-qPCR analysis (B–E) were performed to examine the mRNA levels of ISG15 (B), IFITM1 (C), IFITM3 (D), and OASL (E). (F–H) 293T cells were transfected with 3 μg I226R-Flag plasmid or EV for 24 h; then, they were transfected with 1 μg/mL of poly(I:C) for 12 h. RT-qPCR analysis was performed to examine the mRNA levels of IFITM1 (F), IFITM3 (G), and OASL (H). (I–K) 293T cells were co-transfected with 2 μg I226R-Flag plasmid or EV and 500 ng cGAS-Flag and 100 ng STING-HA plasmids. RT-qPCR analysis was then performed to examine the mRNA levels of IFITM1 (I), IFITM3 (J), and OASL (K) 24 h later. The results are expressed as the means ± standard deviation from three independent experiments. Statistical analysis was performed using the Student’s t-test. * p < 0.05; ** p < 0.01.