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. 2022 Mar 8;27(6):1763. doi: 10.3390/molecules27061763

Figure 1.

Figure 1

Time-dependent effect of TLR3/4 stimulation on CXCL10, IFN-β, and TNF-α expression in PBMCs from healthy volunteers. Time-dependent (2–24 h) effect of poly(I:C) (10 μg/mL) treatment on the protein expression of (a) CXCL10 (Kruskal–Wallis statistic = 14.4), (c) IFN-β (Kruskal–Wallis statistic = 20.6), and (e) TNF-α (F(5,24) = 0.33; ANOVA) in PBMCs. Time-dependent (2–24 h) effect of LPS (100 ng/mL) treatment on the protein expression of (b) CXCL10 (Kruskal–Wallis statistic = 3.98), (d) IFN-β (Kruskal–Wallis statistic = 13.4), and (f) TNF-α (F(5,24) = 6.16; ANOVA) in PBMCs. Data were tested for normality using the Shapiro–Wilk test and statistical significance determined by Kruskal–Wallis tests, with Dunn’s multiple comparison post-hoc test (for (a–d)), or one-way ANOVA with Dunnett’s multiple comparison test (for (e,f)). Data are presented as median cytokine/chemokine expression, minimum and maximum values and IQR. Symbols indicate individual data points from HCs (n = 5–11). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. cells treated with media alone.