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. 1999 Oct;43(10):2538–2541. doi: 10.1128/aac.43.10.2538

FIG. 1.

FIG. 1

Cointegrate formation involving R388 attI1 site (Inline graphic) and aadB 59-BE (Inline graphic) on pSU1817. Note that the attI1 site flanking aadB on pSU1817 is incomplete. The double-headed arrow indicates sites involved in the formation of cointegrates. BamHI (B) and HindIII (H) sites are shown. (a) Recombination between R388 attI1 site and 3′ core site of 59-BE on pSU1817; (b) recombination between R388 attI1 site and 3′ core site of 59-BE on pSU1817 and excision of orfA gene cassette; (c) recombination between R388 attI1 site and 3′ core site of 59-BE on pSU1817 and excision of orfA and dfrB2 gene cassettes. Note that the same result would also be produced by recombination between the 59-BE of orfA of R3888 and aadB of pSU1817, combined with the excision of the orfA and dfrB2 cassettes. pSU18 is indicated by a broken line. Sizes below maps are in kilobases.