Table 2.
Comparison of the amino acid substitutions between the Spanish isolates and representatives of the Lombardy and Veneto clusters of WNV-L2. The isolate from Hungary 2004 was used as the reference sequence. Black dot indicates the same amino acid as the reference sequence. Amino acids that are unique in Spanish WNV-L2 polyproteins are highlighted in bold.
| Initial Reference | Veneto Cluster | Lombardy Cluster | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Viral Protein | Amino Acid Position |
DQ116961 Hungary 2004 (Goshawk) |
KF647249 Italy 2013 Rovigo (Human) |
KF823806 Italy 2013 Mantova (Human) | MT863560 France 2018 (Common Buzzard) | AC568 Spain 2017 Lleida (Northern Goshawk) | AC913 Spain 2020 Lleida (Northern Goshawk) | AC923 Spain 2020 Tarragona (Northern Goshawk) | AC924 Spain 2020 Tarragona (Northern Goshawk) |
| C | 34 | M | · | · | · | · | · | V | · |
| prM | 20 | T | A | · | · | · | · | · | · |
| 29 | A | · | · | · | · | · | · | V | |
| E | 88 | S | P | P | P | P | P | P | P |
| 159 | I | T | T | T | T | T | T | T | |
| NS1 | 35 | Y | · | H | H | H | H | H | H |
| 41 | G | · | · | · | · | S | · | · | |
| 69 | G | E | E | E | E | E | E | E | |
| 92 | K | · | · | E | · | · | · | · | |
| 113 | M | · | · | · | · | · | I | · | |
| 123 | I | · | · | · | F | F | F | F | |
| 146 | A | V | · | · | · | · | · | · | |
| 293 | H | · | · | · | R | R | R | R | |
| NS2A | 1 | H | Y | Y | Y | Y | Y | Y | Y |
| 192 | S | C | C | C | C | C | C | C | |
| NS4B | 11 | N | · | · | · | · | · | S | S |
| NS5 | 26 | A | T | T | T | T | T | T | T |
| 203 | Y | H | · | · | · | · | · | · | |
| 277 | T | · | · | · | I | I | I | I | |
| 279 | K | · | R | · | · | · | · | · | |
| 299 | A | T | T | T | T | T | T | T | |
| 340 | N | S | · | · | · | · | · | · | |
| 599 | R | · | · | · | · | · | · | I | |
| 886 | V | A | · | · | · | · | · | · | |