TABLE 2.
Direct detection of M. tuberculosis rpoB in 84 clinical specimens collected from 62 patients suffering from tuberculosis
| Specimen source (no. of specimens)a | Resultb
|
|||
|---|---|---|---|---|
| Smear | Culture | IS6110 PCRc | rpoB PCR | |
| Respiratory (39) | + | + | + | + |
| Respiratory (5) | − | + | + | + |
| Respiratory (1) | − | − | + | + |
| Respiratory (15) | + | + | + | − |
| Respiratory (7) | − | + | + | − |
| Respiratory (5) | − | − | + | − |
| Nonrespiratory GW and L (6) | + | + | + | + |
| Nonrespiratory GW, PE, and U (3) | − | + | + | + |
| Nonrespiratory GW and L (2) | + | + | + | − |
| Nonrespiratory PE (1) | − | − | + | − |
a
Respiratory specimens include sputum and bronchial wash or lavage specimens. Abbreviations: GW, gastric wash; L, lymph node; PE, pleural effusion; U, urine.
b
+, positive; −, negative.
c
A previously described homemade nested PCR protocol was used to obtain these results (16).