Table 4.
LC method validation parameters.
| Paramethers | Ketamine | Ketamine * | Midazolam | Midazolam * | Loxapine | Loxapine * | Clonidine | Clonidine * | Sufentanil | Sufentanil * |
|---|---|---|---|---|---|---|---|---|---|---|
| Linearity | ||||||||||
| Concentration range (µg mL−1) | 1 to 10 | 1 to 10 | 5 to 50 | 5 to 50 | 5 to 50 | 5 to 50 | 21 to 39 | 21 to 39 | 0.7 to 1.3 | 0.7 to 1.3 |
| Slope | 2.78 | 2.52 | 3.82 | 4.26 | 4.21 | 4.20 | 6.16 | 6.21 | 14.44 | 14.41 |
| SD Slope | 0.04 | 0.042 | 0.03 | 0.01 | 0.09 | 0.09 | 0,08 | 0.07 | 0.68 | 0.45 |
| y-intercept | −1.41 | −0.87 | −14.15 | −8.66 | −20.01 | −22.23 | 1.40 | 3.80 | 19.67 | 23.04 |
| SD y-intercept | 0.22 | 0.25 | 0.77 | 0.23 | 2.88 | 2.68 | 2.36 | 2.28 | 0.69 | 0.46 |
| Correlation coefficient (R) | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.96 | 0.98 |
| LOD (µg mL−1) | 0.30 | 0.30 | 1.0 | 1.0 | 1.0 | 1.0 | 0.20 | 0.20 | 0.002 | 0.002 |
| LOQ (µg mL−1) | 0.90 | 0.90 | 3.0 | 3.0 | 3.0 | 3.0 | 0.60 | 0.60 | 0.006 | 0.006 |
| Accuracy | ||||||||||
| %Recovery | 99.14 | 101.26 | 99.60 | 99.75 | 97.64 | 100.67 | 100.07 | 99.93 | 99.97 | 99.96 |
| SD | 4.62 | 3.88 | 2.74 | 1.34 | 2.49 | 3.23 | 1.20 | 1.01 | 4.34 | 3.09 |
| Precision | ||||||||||
| Repeatability | 3.17% | 1.46% | 1.68% | 0.73% | 1.31% | 2.40% | 1.55% | 2.36% | 2.33% | 2.68% |
| Reproducibility | 2.89% | 1.33% | 1.54% | 0.67% | 1.50% | 3.10% | 1.42% | 2.15% | 2.13% | 2.45% |
* in presence of the other molecules.