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. 2022 Mar 16;23(6):3184. doi: 10.3390/ijms23063184

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Concentration response curves of BRET-based responses in HEK293T cells upon stimulation with various H₁R agonists. BRET measurements in HEK293T cells transiently co-expressing H₁R (0.5–1.0 µg plasmid/dish) in combination with G_q activation sensor (2.5 µg plasmid/dish) (A), InsP₃ (B) or Ca²⁺ (C) sensor (4.0 µg plasmid/dish), or H₁R-Rluc8 (1.0 µg plasmid/dish) in combination with GRK2-mVenus (D), GRK3-mVenus (E), GRK5-mVenus (F), GRK6-mVenus (G), β-arrestin1-eYFP (H), or β-arrestin2-mVenus (I) (4.0 µg plasmid/dish), upon stimulation with increasing concentrations agonist for 20 (A–C)or 60 min (D–I). Data are shown as mean ± SD from at least three independent experiments performed in triplicate.