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. 2022 Mar 16;23(6):3184. doi: 10.3390/ijms23063184

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Activities of agonists in endogenous H₁R-mediated Ca²⁺ response in HeLa cells. Concentration response curves of Ca²⁺ mobilization measured 20–30 s after stimulation of HeLa cells with agonists (A). The pEC₅₀ (B), intrinsic activity (C), transduction ratio (Log(τ/K_A) (D), and relative effectiveness (ΔLog(τ/K_A) as compared to reference agonist histamine (E) of agonist-induced Ca²⁺ mobilization in HeLa cells are shown as mean ± SD from at least three independent experiments performed in triplicate.