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. 2022 Mar 11;8(3):292. doi: 10.3390/jof8030292

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Assay for lipid homeostasis in B. bassiana. The lipidomic assay indicated that the ablation of BbSAY1 resulted in the impaired homeostasis of a neutral lipid (A) and phospholipid (B). (C) Sytox-green nucleus staining. The ΔBbsay1 mutant strain displayed anincreased cytomembrane permeability, and its nuclei were easily stained by fluorescent dye. (D) Staining lipid bodies. The fluorescent dye BODIPY was used to stain the lipid bodies in fungal cells. The ΔBbsay1 mutant strain accumulated the enlarged lipid bodies, which indicatedthe unbalanced lipid metabolism after the ablating of BbSAY1.