Figure 3.

Andrographis enhances the efficacy of berberine in mouse xenografts and patient-derived tumor organoids. (A) A schematic diagram of the RKO xenograft model in nude mice and the treatment schedule of berberine, Andrographis, and their combination; (B) The bodyweight alterations of the mice in each treatment group were measured at different time-points after inoculation; (C) The tumor volume alterations of mice in each treatment group were measured at different time-points after inoculation; (D) Representative images of anatomic tumor xenografts dissected from nude mice after they were sacrificed; (E) The measurement of the xenograft weights of each treatment group; (F) Microscopic images of patient-derived tumor organoids derived from two patients cultured in each treatment group (20× magnification). The organoid count analysis is shown in the right panel. Statistical Significance: * p < 0.05, ** p < 0.01, *** p < 0.001. BBR, berberine; Andro, Andrographis.