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Schematic representation of the aptasensor design and its working principle: after the electrochemical deposition of AuNPs streptavidin was immobilized on the working electrode, then a solution of BSA and sorbitol was used to block the surface; subsequently, the biotinylated aptamer was immobilized, and after a final step of blocking with biotin, the sensor was ready to use. The redox probe [Fe (CN) 6]3−/[Fe (CN) 6]4− was employed to evaluate the presence or absence of PWG-Gliadin.
