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. 2022 Mar 12;14(3):585. doi: 10.3390/v14030585

Figure 17.

Figure 17

Electron microscopy and histological changes in shrimp infected with yellow head virus genotype 1 (YHV). (A) TEM of negative-strained YHV virions (Scale bars = 100 nm); (B) LO tissue of moribund shrimp from YHV immersion challenged P. vannamei at day 5 showing numerous pyknotic nuclei (arrows), karyorrhectic nucleic and cytoplasmic inclusion (arrow heads); (C) Hemolymph from normal and YHV infected shrimp identified by staining hemolymph smears; (D) Gills of YHV infected shrimp stained with H&E in rapidly fixed and stained (3 h) whole mounts. ((A) Reprinted from Advances in virus research, Vol. 63, Dhar, A.K., Cowley, J.A., Hasson, K.W., Walker, P.J., Genomic organization, biology, and diagnosis of Taura syndrome virus and yellow head virus of penaeid shrimp, p. 69, Copyright (2004), with permission from Elsevier; (B) Reprinted from Developmental & Comparative Immunology, Vol. 32 (6), Anantasomboon, G., Poonkhum, R., Sittidilokratna, N., Flegel, T.W., Withyachumnarnkul, B., Low viral loads and lymphoid organ spheroids are associated with yellow head virus (YHV) tolerance in whiteleg shrimp Penaeus vannamei, p. 14, Copyright (2008), with permission from Elsevier; (C,D) Reprinted from Aquaculture, Vol. 258 (1–4), Flegel, T.W., Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand, p. 33, Copyright (2006), with permission from Elsevier).