FIG. 2.

Accumulation of [³H]dihydrostreptomycin by intact cells of P. aeruginosa PAO1 (⧫) and mutant 11B (▴). The MICs of dihydrostreptomycin for PAO1 and mutant 11B were 16 and 4 μg ml⁻¹, respectively. The cells were grown in LB, harvested, washed (50 mM phosphate buffer, 100 mM LiCl), and resuspended in 50 mM of phosphate buffer (pH 7) containing 1 mM MgSO₄ and 0.2% (wt/vol) glucose as a source of carbon. After 5 min at 37°C, a mixture of dihydrostreptomycin and [³H]dihydrostreptomycin (20 Ci mmol⁻¹) was added to a final concentration of 8 μg ml⁻¹; and the samples were taken and filtered, and the radioactivity was counted. The data are means for three independent experiments.