Skip to main content
. 2021 Apr 23;118(5):1276–1288. doi: 10.1093/cvr/cvab149

Figure 2.

Figure 2

Cardiomyocyte-specific EGFR downregulation induces delayed cardiac remodelling. Cardiac structure was assessed in the LV of 10-month-old EGFRf/f (Cre−) vs. CM-EGFR-KD mice via WGA (A), TUNEL/TNNI (B), and MTC (C) staining. Panels shown are representative images from n = 6 (EGFRf/f (Cre−)) and n = 5 (CM-EGFR-KD) mouse hearts; scale bars denote 100 µm and arrowheads denote TUNEL-positive cardiomyocytes. Quantification of cardiomyocyte size, death, and cardiac fibrosis are shown in accompanying histograms. ***P < 0.001, two-tailed unpaired t-test. (D) RT-qPCR was used to measure the expression of Acta1, Myh7, Nppa, Il6, and Tnfa in the LV of 10 month-old EGFRf/f (Cre−) (n = 5) vs. CM-EGFR-KD (n = 6) mice. P < 0.05, **P < 0.01, two-tailed unpaired t-test. %EF (E) and %FS (F) of 10-month-old EGFRf/f (Cre−) (n = 10) and CM-EGFR-KD (n = 7) mice were measured via echocardiography. ***P < 0.001, two-tailed unpaired t-test. (G) EGFRf/f (Cre−, n = 19) and CM-EGFR-KD (n = 28) mice were monitored for survival over time. ***P < 0.001 vs. EGFRf/f (Cre−), Log-rank (Mantel–Cox) test.