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. 2021 Apr 23;118(5):1276–1288. doi: 10.1093/cvr/cvab149

Figure 6.

Figure 6

Cardiomyocyte-specific EGFR downregulation alters Ca2+ reuptake. (A) LV cardiomyocytes were freshly isolated from adult EGFRf/f (Cre−) vs. CM-EGFR-KD hearts, loaded with fura2 and stimulated at 2 Hz and Ca2+ transients measured at baseline (black line) and after stimulation with ISO (0.1 μM, red line), as shown in tracings representative of n = 23 (EGFRf/f (Cre−)) and n = 22 (CM-EGFR-KD) individual cardiomyocytes isolated from n = 3 mice each, with quantification of peak Ca2+ transients (B) and time to 50% Ca2+ clearance (C) summarized in histograms. ns, not significant, *P < 0.05, ***P < 0.001, ordinary one-way ANOVA with Tukey’s multiple comparisons test. Immunoblot analysis of adult EGFRf/f (Cre−) vs. CM-EGFR-KD cardiac LV lysates, as summarized in histograms, for Ser16 phospholamban (P-S16-PLB, D, n = 7 each) and Thr17 phospholamban (P-T17-PLB, E, n = 8 each). *P < 0.05, **P < 0.01, two-tailed unpaired t-test.